The RCM is a standard confocal microscope extended with a re-scan detection unit. With a simple trick a resolution of 170nm can be achieved for any pinhole diameter. The pinhole is only needed for Z-sectioning. Since RCM has also a strongly improved sensitivity there is no need for high laser power, being more friendly for cells. At this moment RCM can work in multi-colour mode for different colour combinations and ratio-imaging. But it is our mission to go further by extending the spectral range into the IR for deep tissue imaging and real-time acquisition speed for monitoring highly dynamic processes. Applications like FRET, FRAP, pH, and Ca2+ are possible and many more biological applications are on the horizon.
The RCM unit is a plug-and-play box that can easily be connected via standard C-mount adapters to the microscope and camera. The laser light can be fed into the RCM unit via a single mode fibre connector. All standard connectors, no restrictions here!
The complete solution contains the RCM Unit, an inverted Nikon microscope, laser source up to 4 wavelength and a Hamamatsu Orca Flash4 sCMOS camera. The system currently sold together with the Nikon imaging software but the Labview-based, self developed solution will be released soon (planned date is April).