Femto3D-Atlas
Overview
Femto3D Atlas is an acousto-optic scanner based two-photon microscope extension which can be added to existing upright microscopes to open their imaging capability to fast 3D functional imaging. It contains two pairs of AO deflectors (AODs) which are responsible for the X, Y, and Z focusing. The unique property of this scanner type that it does not contain scanning mirrors or any other slowly moving mechanical components, so the positioning of the focal spot is fast, flexible, stable and independent of the travelling distance. This positioning freedom results an extremely high scanning speed, up to 30 kHz at any 3D location under the objective in a cubic millimeter volume.
3D imaging
The novel features of these microscopes are the electrically tunable acousto-optic (AO) deflectors, which do not contain scanning mirrors or any other slowly moving mechanical components, so the positioning of the focal spot is fast, flexible, stable and independent of the travelling distance. This positioning freedom results an extremely high scanning speed, up to 30 kHz at any 3D location under the objective in a cubic millimeter volume.
- -generation acousto-optics provides over 4-fold improved excitation
- Automatic beam stabilization
- Angular dispersion compensation
- Multiple wavelengths and Acousto-optic deflectors
The highest technology
In contrast to a traditional scanner, the Femto3D AcoustoOptic microscopes contain two pairs of AO deflectors (AODs) which are responsible for the X, Y scanning, and Z focusing. The AODs control the optical beam spatially, utilizing the interaction between sound and light waves. In imaging, AODs diffract laser beam through ultrasonically generated gratings, and sound waves induce pressure fluctuation in the AOD crystal. By changing the sound frequency, the focus point also changes. The operation of the AOD is silent, it does not disrupt the experiment and the animal sample. The integrated electric stabilization of the 3D scanner provides long-term stability in measurements without the need for maintenance.
Features
- 30 kHz/ROI scanning speed in 3D
- In vitro measurements and Mightex deep tissue imaging over 650 μm
- Over 500 μm × 500 μm × 650 μm scanning volume
- New automatic wavelength tunability
- Dynamic compensation for optical errors
- Intelligent software control
Benefits
- Novel 3D scanning methods both for in vivo and in vitro
- Flexible ROI scanning possibilities
- Fast recording of over 2000 soma distributed in 3D
- Imaging of dendritic branches without interruption
- Simultaneous measurements of thousands of spines with high SNR
- Motion correction in behaving animals
- Improved in total excitation efficiency for genetically encoded calcium indicators
- No mechanical vibration induced noise
- High sensitivity, bright signals
- A vast of measurement and analysis possibilities
Femto3D Omega
Overview
Femto3D Omega is our stand-alone, complete acousto-optic scanner-based microscope. It was the first two-photon microscope on the market which used acousto-optic scanner enabling the researchers to image thousands of neurons being in distributed locations of the living brain. The first Femto3D Omega was installed nearly 10 years ago. Since then, many breakthrough results have been published in high-impact journals such as the Nature or Neuron
Features
- the fastest 3D scanning methods
- 30 kHz scanning speed
- all 3D scanning methods featured Femto3D AcoustoOptic product line
- simultaneous imaging of 2000< neurons for network imaging
- dendritic imaging resolving activity of single action potentials
- for in vitro, in vivo and behavior studies
