The FemtoSMART is a resonant and galvanometric scanner based two-photon microscope product line, which allows the in vivo imaging.
FemtoSmart Galvo
Technology Overview
The FemtoSmart Galvo is a galvanometric scanner-based two-photon microscope which enables in vivo and in vitro functional imaging to be focused on the region of interest (ROI). The scanner consists of two galvanometer-based motor-driven mirrors, meaning that the focal spot can be positioned where required. The high accuracy and this positioning freedom support flexible approaches for ROI creation. In contrast to sampling all pixels in images, ROI scanning (e.g. along a predefined line) restricts scanning to the regions which are relevant for the scientist, resulting in faster recording, and elimination of background noise. This helps to reveal cellular signaling and action potentials.
Specification
- in vivo deep brain imaging down to 850 µm
- 800 µm × 800 µm FOV (with a 20x obj.)
- 2D scanning modes
point and random access point with 200 µs/point
free hand line and multiple line: 40 lines/5 ms
frame with 4.1 fps at 512 x 512 pixel, 750 µm x 750 µm
folded frame, multiple folded frame - pixel dwell time adjustable: 0.5 µs - 10 ms, pixel-based averaging
- minimized optical path length by patented travelling detector system
- non-descanned, ultrasensitive GaAsP PMT
- high signal-to-noise ratio
- simultaneous detection of multiple wavelength
- custom-designed optical elements for maximal transmission efficiency
- MATLAB-based control software with analysis and upgrade possibilities
- ∆F/F, ∆G/R calculation
- parallel recording and analysis of electrophysiological data
- CMOS camera
- compatibility with extended IR wavelength range
FemtoSmart Resonant
Overview
In the FemtoSmart Resonant microscope, Femtonics combines high-speed and high-sensitivity imaging of living tissues by using a fast resonant scanner. Resonant-scanner-based raster scanning acquires images of the entire field-of-view ~5 times faster than galvanometric-based scanning: it is therefore the most appropriate choice for imaging the entire field-of-view at high frame rate.
Specification
- in vivo deep brain imaging down to 850 µm
- 600 µm × 600 µm FOV (with a 20x obj.)
- scanning modes
raster scanning with 31 fps at 512×512 pixels and 500 fps at 512×32 pixels
16 000 lines/sec (straight, parallel with x-axis)
3D volume scanning with 3 Hz by Piezo objective positioner - dynamic pixel dwell time to avoid image distorsion
- minimized optical path length by patented travelling detector system
- non-descanned, ultrasensitive GaAsP PMT
- high signal-to-noise ratio
- simultaneous detection of multiple wavelength
- custom-designed optical elements for maximal transmission efficiency
- C++-based control software with analysis and upgrade possibilities
- dedicated software efficiently handling large datasets
- nearly unlimited measurement times
- ∆F/F calculation
- parallel recording and analysis of electrophysiological data
- CMOS camera
- compatibility with extended IR wavelength range
Applications
- For in vivo high-speed functional imaging in deep tissues
- Rapid image acquisition
- Appropriate choice for imaging the entire field-of-view
- Long-term measurements
- Time-lapse imaging
- 3D volume scanning upgrade
- Intelligent control software
FemtoSmart Dual
Technology Overview
The FemtoSmart Dual microscope contains both galvanometric and resonant scanners, providing all advantages of the two types of imaging technique. Using the two scanners function in tandem is a perfect solution for photo stimulation. The galvanometric scanner directs the laser to cells or subcellular components selectively. The selectivity is established by scanning along arbitrary line patterns placed on cell bodies or dendritic segments. With the resonant scanner, you can follow the changes in the cells of a neural network collecting imaging data simultaneously by high-speed frame scanning of the surrounding area. Timing of photo stimulation and gating of the detectors are controlled by protocols designed on user-friendly GUI.
Specification
- in vivo deep brain imaging down to 850 µm
- properties of the galvanometric scanner based imaging
800 µm × 800 µm FOV (with a 20x obj.)
point and random access point with 200 µs/point
free hand line and multiple line: 40 lines/5 ms
frame with 4.1 fps at 512 x 512 pixel, 750 µm x 750 µm
folded frame, multiple folded frame
pixel dwell time adjustable: 0.5 µs – 10 ms, pixel-based averaging - properties of resonant scanner based imaging
600 µm × 600 µm FOV (with a 20x obj.)
raster scanning with 31 fps at 512×512 pixels and 500 fps at 512×32 pixels
16 000 lines/sec (straight, parallel with x-axis)
3D volume scanning with 3 Hz by Piezo objective positioner
dynamic pixel dwell time to avoid image distorsion - custom-designed optical elements for maximal transmission efficiency
- non-descanned, ultrasensitive GaAsP PMT
- minimized optical path length by patented travelling detector system
- control software with analysis and upgrade possibilities
- high signal-to-noise ratio
- simultaneous detection of multiple wavelength
FemtoSmart Bridge
Technology Overview
The FemtoSmart Bridge is a special edition of the FemtoSmart microscope which is designed to provide extreme freedom in positioning of the body. It involves all the advantages of the FemtoSmart series but it has been extended, making it particularly beneficial to studies which need a very large space for the sample or accessories by enabling functional brain imaging in head-fixed mice navigating in a large mobile cage.
Unique features
- Z moving range by the lifting apparatus: 50 cm
- Coarse Z adjustment: 1 mm
- Z moving range by the by the objective holding arm: 50 mm
- Fast Z positioning by Piezo objective positioner kit: 400 µm
- XY actuator moves the body relative to the column in the X and Y directions: ±25 mm range, step size: 1 µm
- Our Tilting objective kit further increases accessibility to organs