OASIS – Optical Activation, Stimulation and Imaging System
Ground-breaking platforms for in vivo optogenetics and Ca2+ -imaging.
OASIS Implant
Freely-Behaving Imaging and Optogenetics
Understand the Link Between Neural Circuits and Behaviour
The OASIS Implant is a ground-breaking platform for simultaneous cellular-resolution optogenetics and calcium imaging in freely-behaving animals to probe complex neuronal networks in the deep-brain, cortex, and multiple brain regions, or spinal cord.
- Freely-Behaving Calcium Imaging and Optogenetics
- Cellular-Resolution Optogenetics
- Multi-Region Investigation
- Reconfigurable Platform
- High-Quality Imaging with Scientific Cameras
To offer neuroscientists the ability to stimulate and visualize individual neurons in freely-behaving animals, Mightex’s engineers went back to the drawing board and pioneered a new path in the in vivo calcium imaging field. Instead of miniaturizing and placing the microscope on the animal – thereby limiting the microscope’s features and the animal’s behaviour – we kept the microscope out of the animal’s head by making use of long and flexible imaging fibers.
How It Works
By keeping the microscope away from the animal’s head, the OASIS Implant accomplishes 2 main goals:
The Only Reconfigurable Calcium Imaging Platform
The OASIS Implant is a modular platform that can be reconfigured to be able to perform different experiments as the need arises. Transition from single- to multi-wavelength experiments, or from calcium imaging to targeted optogenetics within one single system using the OASIS Implant.
Front View Back View
Intuitive Signal Processing for Data Analysis
Every OASIS Implant system comes with Mightex’s Image Acquisition and Analysis software that allows users to collect large data sets of neural recordings from calcium imaging experiments and provides the key processing and analysis tools required to help users quickly convert these large data sets into results that can be clearly interpreted for analysis.
1. Acquire In Vivo Calcium Recordings
The software provides a complete set of tools to allow researchers to visualize and capture calcium imaging recordings. The software includes features to synchronize the calcium recordings with the rest of the behavioral equipment and cameras used in the experiment.
2. Identify Cells
Once calcium recordings are acquired, researchers are able to instantly view and process the captured data. The cell identification tools allow for easy detection of cells and sub-cellular features.
3. Extract DeltaF/F Traces
Traces of the calcium imaging data can be extracted for each cell that is identified. Along with the cell traces, all inputs and outputs to behavioural equipment/cameras will be contained in collected data sets to allow for easy matching of neural events with behavioral activity.
Headmounts Designed for Distinct Applications
Deep-Brain
This headmount is designed for deep-brain applications by coupling the fiber and implanted GRIN lens.
- The lightest and smallest head mount in the market.
- Up to 3X lighter than miniscope alternatives.
- Provides focusing adjustment and ability to lock both focusing and orientation.
Large Field of View
This headmount is designed for large field of view applications (up to 2mm) across the cortex by using a fiber with an increased field of view.
- The lightest and smallest head mount in the market.
- Up to 3X lighter than miniscope alternatives.
- Provides focusing adjustment and ability to lock both focusing and orientation.