by Varjú Patrícia

Single Cell Isolation

CellSorter develops smart technologies for single cell handling, sorting, and isolation.

Robotic Isolation Technology to discover single cell genetics

Full CellSorter Instrument with Valve Control

  • High throughput single cell sorting directly from the Petri dish
  • One single cell arrives to each PCR tube
  • 10 PCR strips containing 80 tubes can be filled in a cycle
  • Glass cover slip for testing single cell deposition in situ
  • Drop volume less than 1 ul for adherent cells
  • Pick up volume of ~1 nl for suspended cells
  • 15-20 seconds per cell. When collecting multiple cells, sorting speed is 1 cell/second.
  • Number of cells picked up in a single run: 1-1000.
  • Isolates a subpopulation of live adherent cells expressing fluorescent or luminescent markers
  • Both unlabeled and fluorescentcells are recognized by computer vision
  • Viable cells after sorting
  • Any adherent and non-adherent cell type can be sorted
  • Cell culture needs minimal preparation before sorting
  • Average sorting process takes only a few minutes
  • Multichannel detection using the fluorescent filter setup of the microscope


Valve Controlled CellSorter Device & Software

  • High precision micropipette holding console made from hard aluminum equipped with LED illumination for micropipette focusing connected to the motorized micromanipulator via a rotatable arm.
  • 25 mm travel range 1D motorized micromanipulator
  • Special aluminum insert fitting to the microscope stage with a hole and magnetic fixing ring for the 35 mm Petri dish and 80 holes for 10 PCR stripes and a hole for a 32×24 mm2 glass cover slide for testing and high resolution imaging and a hole with a calibration cross-hair
  • CellSorter control unit with 2 high speed fluid valves and USB connection to the computer
  • User friendly intuitive control software for MS Windows OS
  • Accessories, fittings and tubing for liquid control
  • 5 pcs of borosilicate micropipettes for single cell manipulations with calibrated aperture

Motorized Zeiss Axio Observer A1 Inverted Fluorescent Microscope

  • Including NA 0.55 LD condensor lens, EC Plan neofluar 10x/0.3 Ph 1 objective lens
  • Accessories for phase contrast and fluorescent imaging
  • Fluorescent illumination system with Prior Lumen 200S metal halide light source
  • Marzhauser 2D motorized microscope stage and motorized focus drive.

Andor Zyla 5.5 Camera

  • USB 3.0 sCMOS – Compact low light imaging camera
  • Front-illuminated scientific CMOS sensor
  • 2560 x 2160 pixel array, 6.5 μm pixel size
  • Low read noise of 1.2 e-, sensor stably cooled to 0°C up to an ambient temperature of +35°C
  • Rolling shutter and ‘true’ global shutter modes
  • 12 bit and 16 bit digitization
  • Max. pixel readout rate 2x280MHz
  • iCam, C-mount, USB3.0 interface (interface card and cable included), power supply, trigger cable

NE-1000 Syringe Pump

  • Holds 1 Syringe up to 60 cc
  • Infusion rates from 0.73 µL/hr (1 cc syringe) to 2100 ml/hr (60 cc syringe)
  • Easy-to-use keypad interface
  • Space Saving Chassis: Foot print size of only 5 3/4″ x 8 3/4″
  • Includes all the advanced functions and programming features of the NE-1000 family

 Dell Desktop Computer With 23″ Dell Monitor

  • Processor: Intel Core i7-4790 3,6: 4 Cores, 8M Cache, up to 4.00 GHz
  • Memory: 8 GB DDR RAM
  • HDD: 1000 GB
  • SSD: 120 GB

Valve Controlled Cellsorter Device & Software can be ordered separately.

New Piezoelectric Sorting Head for Single Cell Isolation


  • Compact design
  • Excellent liquid handling precision: 0.1 nanoliter
  • Outstanding >90% single cell isolation efficiency
  • Built-in LED illumination for phase contrast imaging
  • No tubes
  • No syringes
  • control unit with USB connection to the computer