Analyze force spectroscopy data including force-distance curves and force volume measurements. Quantify adhesion, nanoindentation, and model molecular unfolding with WLC fitting.
⇒ Automated pre-processing of force curves – incoming force curves are automatically processed: denoised (using median or Gaussian filters), baseline-corrected, and calibrated based on cantilever and spring constant values.
⇒ Flexible force curve visualization – display approach and/or retraction segments. Choose axis units:
X-axis: distance, separation, or time
Y-axis: deflection (in volts or nanometers), or force (in nN)
⇒ Automatic detection of adhesion events – adhesion points are identified and displayed automatically, with the option to fine-tune the attraction and retraction points manually. Extract key parameters:
Distance and force at event
Slope
Distance and force difference
⇒ Wormlike chain (WLC) modeling – fit WLC models to force curves representing protein unfolding. Calculate persistence length, contour length, and the effective spring constant for each unfolding event.
⇒ Batch analysis of force curves – view all curves in a series as a grid, scroll through them individually, and display parameters for each. Generate statistical outputs like control charts, scatter plots, and histograms for selected parameters.
⇒ Force volume visualization – create an interactive map representing parameter values calculated for each pixel or point in a force volume dataset.
⇒ Force volume filtering and extraction – denoise and smooth the dataset, then interactively extract slices or individual force curves for closer analysis.
Included in:
- MountainsSPIP® Premium
- MountainsLab® Premium
Available with:
- MountainsSPIP® Starter
- MountainsSPIP® Expert
- MountainsSpectral® Expert
- MountainsSpectral® Premium
- MountainsLab® Expert
