SUPERNOVA‑3000 – High-Speed, High-Resolution Miniature Two-Photon Imaging System
Breakthrough Imaging for Fast Neural Dynamic
The SUPERNOVA‑3000 is a next-generation miniature two-photon imaging system optimized for ultra-fast imaging of neural activity in freely behaving animals. Built on Transcend’s proven miniature platform, it offers unprecedented imaging speed and resolution—making it ideal for capturing fast calcium transients, voltage signals, and dynamic processes in real time.
High-Speed, High-Resolution Data
Equipped with advanced scanning and high-sensitivity detection, the system captures up to 100 frames per second while maintaining subcellular resolution. It supports multiple excitation wavelengths (780, 920, and 1030 nm), and offers a wide field of view—making it suitable for visualizing fast calcium or voltage signals across extended cortical areas.
Key features os Transcend Supernova-3000
Go Deeper
Long-wavelength femtosecond lasers (e.g. 1320 nm) enable calcium imaging at depths up to 1.2–1.4 mm. This allows full visualization through the cerebral cortex and corpus callosum, reaching deep brain regions such as the hippocampus in mice. Deep imaging can be performed safely using only cranial window surgery, reducing tissue damage compared to implanted GRIN lenses.
More Freedom
The system’s 2.2 g miniature headpiece is easily mounted on small animals, enabling naturalistic behavior during imaging. Its novel optical architecture integrates flexible light-shielding fibers, hollow-core fiber optics, MEMS scanners, and ETL-based 3D focusing—allowing full volumetric imaging with minimal footprint.
Less Damage
The optical configuration minimizes phototoxicity by using longer wavelength excitation light and improving scattered fluorescence collection. This permits deep imaging with lower laser power, ensuring stable, high-quality data over extended sessions while protecting delicate brain structures.
Integrated Optogenetics and Dual-Wavelength Imaging
SUPERNOVA‑3000 is fully compatible with optogenetic stimulation, allowing simultaneous two-photon imaging and light-based manipulation of targeted neurons. Dual-laser operation (e.g. 920 nm imaging + 635 nm stimulation) enables synchronized recordings and causal experiments in behaving animals. This opens new avenues for understanding functional circuits with precise spatial and temporal control.
Advanced 3D Brain Imaging
SUPERNOVA‑3000 enables precise 3D reconstruction of mouse brain tissues in awake, head-fixed animals. It supports imaging of blood vessels and neurons from the cortex to the hippocampus, including key areas like the CA1 subregion and postsubiculum. Using dyes and indicators such as FITC-Dextran, AAV-GCaMP6, or Thy1-YFP, researchers can visualize neural anatomy and activity from 0 to over 1400 μm depth with excellent resolution.
3D reconstruction imaging of blood vessels from the mouse cortex to the hippocampus region
Green: FITC-Dextran dye
Imaging depth: 0~1428 μm
Excitation wavelength: 1320 nm
Head-fixed imaging of awake mice
3D reconstruction imaging of neurons from the mouse posterior parietal cortex to the hippocampal CA1 subregion
Green: AAV-hSyn-GCaMP6s
Magenta: Third harmonic signal
imaging depth: 0~1236 μm
Excitation wavelength: 1320 nm
Head-fixed imaging of awake mice
3D reconstruction imaging of neurons from the posteriorparietal cortex to the CA1 area of the hippocampus in mice
Green: Thy1-YFPH
Imaging depth: 0~1038 μm
Excitation wavelength: 1320 nm
Head-fixed imaging of awake mice
3D reconstruction of mouse cerebral cortex to postsubiculum
Green: AAV-hSyn-GCaMP6f
Imaging depth: 0~1000 μm
Excitation wavelength: 1320 nm
Head fixed imaging of awake mice
Why Choose SUPERNOVA‑3000?
SUPERNOVA‑3000 offers a rare combination of imaging depth, speed, resolution, and minimal invasiveness in a wearable format. It empowers researchers to:
- Track rapid neural events such as calcium or voltage spikes in real time
- Image deep brain regions like the hippocampus with minimal tissue damage
- Conduct 3D volumetric imaging with ETL-driven focus scanning
- Combine imaging with optogenetic stimulation for causal neuroscience
- Operate reliably in demanding, long-term behavioral experiments
Its modular design ensures scalability, while remote diagnostics and technical support offer peace of mind in long-term research projects.
Step into the future of deep, fast, and flexible in vivo imaging.
Contact us today to request a quote, schedule a demo, or learn how SUPERNOVA‑3000 can advance your neuroscience research.