Confocal RCM2

by Gergely Balogh

Meet RCM2

Brighter images. Larger field of view.

RCM2 is our second generation RCM, with digital scanner technology. It makes bi-directional scanning the standard and allows a speed of 2fps at 512×512 pixels. RCM2 has optics to make it suitable for super-resolution imaging with high NA objectives in the low magnification range, like 40x 1.4. A lower magnification allows for a bigger field of view (FOV), brighter images, and even lower laser power. RCM2 has demonstrated imaging at 10 nano-watt excitation power!

Improvements of sCMOS cameras allow you to sample resolution of low magnification objectives effectively, without increasing the exposure time. In a regular PMT-based confocal this is not possible.

Improve your imaging experience with RCM2

  • Super-resolution (120nm) imaging with 40x 1.4NA objectives.
  • Larger FOV without increasing acquisition time.
    Bidirectional scanner.
  • Excellent add-on to any widefield microscope.
    Easy to use.
  • Hardware integration in third-party software.

See how RCM2 improves your imaging experience for yourself

RCM2: technical specs

 
RCM2
Detector
Camera
Resolution
120 nm **
Sensitivity
up to 95% QE
FOV
220×220 um (40x, super-resolution)
Optimized for
100x, 60x, 40x (high NA)
Scanner
Digital (closed-loop)
Speed
2fps @ 512×512 pixels
Wavelength
VIS
Software
Micromanager, Volocity, NIS Elements, Zen, LAS X, Cellsens
Integration
Hardware – USB connection
PSF for deconvolution with
Microvolution, SVI Huygens
Bypass mode
Yes

*still under investigation
** after deconvolution, raw image = 170nm