The most gentle 3D live cell imaging
AION is the third generation of our fast Line REscan confocal technology. It provides high-contrast images from thicker specimens such as organoids, tissue samples, plant and animal model organisms. The gentle conditions for your live samples allow AION to excel in long-time-lapse experiments and imaging low-signal samples.
The combination of camera-based detection and a set of filters allows you to acquire your images in both visible (VIS) and near-infrared (NIR). This gives you an improved temporal resolution, high sensitivity and unprecedented signal-to-noise ratio. You get even faster imaging with cleaner results, thanks to additional motorized single-band emission filters.
Double the FOV – Double the observation
AION is a confocal system that allows a 25 mm field of view (FOV) with a motorized pinhole that ensures spot-on confocality every time.
Our system uses a line illumination through a slit pinhole, instead of a point illumination found in point scanner confocal microscopes. This provides high temporal resolution while reducing phototoxicity. Since phototoxicity is one of the biggest challenges for researchers, this makes AION the perfect tool for live cell imaging.
You can also add AION to any widefield microscope. This will turn it into an advanced and fast scanning confocal imaging system.
Accelerate your research with the AION
A combination of low laser power requirements, high sensitivity of the detector and a novel optical design present in AION Line REscan enables imaging beyond 500μm of depth. This makes AION Line REscan confocal a perfect solution for live cell confocal microscopy of even thicker specimens like cleared tissue, whole zebrafish embryos, organoids and spheroids.
Explore the benefits
Our flagship AION has a switchable pinhole, ensuring flexibility, sensitivity and optimal confocality.
Nyquisting every objective and adding fast multicolour imaging over a large FOV gives raise to the most light-efficient confocal system on the market.
Discover AION systems that meet your needs
AION α
Detector
Camera (sCMOS)
Resolution in real time
170 nm with deconvolution; 240 nm raw
Detector sensitivity
Up to 96% QE
FOV
25 mm (FN25); 450×450 μm (40x objective)
Speed
>50 fps @ 3Kx3K
Speed in fixed line mode
>1000 fps (camera limited)
Wavelength
VIS+NIR (400-1100 nm)
Software
Micromanager, SDK available for integration on request
Deconvolution
Microvolution (real time); SVI Hyugens (post processing)
Modalities
Brightfield, Widefield and Confocal
Widefield/confocal switch
Motorized
Camera ports
1
Laser fiber inputs
1
Pinhole
Pre-selected
Dichroic
Pre-selected
AION λ
Detector
Camera (sCMOS)
Resolution in real time
170 nm with deconvolution; 240 nm raw
Detector sensitivity
Up to 96% QE
FOV
25 mm (FN25); 450×450 μm (40x objective)
Speed
>50 fps @ 3Kx3K
Speed in fixed line mode
>1000 fps (camera limited)
Wavelength
VIS+NIR (400-1100 nm)
Software
Micromanager, SDK available for integration on request
Deconvolution
Microvolution (real time); SVI Hyugens (post processing)
Modalities
Brightfield, Widefield and Confocal
Widefield/confocal switch
Motorized
Camera ports
2
Laser fiber inputs
2
Pinhole
Motorized
Dichroic
Pre-selected
AION Σ
Detector
Camera (sCMOS)
Resolution in real time
170 nm with deconvolution; 240 nm raw
Detector sensitivity
Up to 96% QE
FOV
25 mm (FN25); 450×450 μm (40x objective)
Speed
>50 fps @ 3Kx3K
Speed in fixed line mode
>1000 fps (camera limited)
Wavelength
VIS+NIR (400-1100 nm)
Software
Micromanager, SDK available for integration on request
Deconvolution
Microvolution (real time); SVI Hyugens (post processing)
Modalities
Brightfield, Widefield and Confocal
Widefield/confocal switch
Motorized
Camera ports
2
Laser fiber inputs
2
Pinhole
Motorized
Dichroic
Motorized